I have designed this high voltage, low current power supply for various experiments in systems & synthetic biology. I have cleaned up the design and I am placing the schematic and board layout online below! This circuit outputs up to +1, 866VDC at under 1 mA or can be tapped at various points for +622VDC or +933VDC. This is useful for either DIY Bi
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ology or institutional research experiments such as: Below is the schematic; read the full post below for the board layout information. Click on the schematic for the full sized version. The schematic operates in stages, so leaving out or bypassing before the 2nd final stage will yield only +933VDC, and leaving out that stage will yield only +622VDC, etc. Imagine reading these kinds of instructions and performing such a task for a few hours: Resuspend pelleted bacterial cells in 250 µl Buffer P1 and transfer to a micro-centrifuge tube. Ensure that RNase A has been added to Buffer P1. No cell clumps should be visible after resuspension of the pellet. If LyseBlue reagent has been added to Buffer P1, vigorously shake the buffer bottle to ensure LyseBlue particles are completely dissolved. The bacteria should be resuspended completely by vortexing or pipetting up and down until no cell clumps remain. Add 250 µl Buffer P2 and mix thoroughly by inverting the tube 4 6 times. Mix gently by inverting the tube. Do not vortex, as this will result in (The protocol examples used here are from Qiagen`s Miniprep kit, QIAPrep. ) Wait a minute! Isn`t that what robots are for Unfortunately, programming a bioscience robot to do a task might take half a day or a full day (or more, if it hasn`t been calibrated recently, or needs some equipment moved around). If this task has to be performed 100 or 10, 000 times then it is a good idea to use...
This project fits more for custom made battery casing that can have more space to hide things inside. This switch with few alterations can be used for any other project as well but is designed based on e-bike needs! This project use a clever functionality of....
The circuit described here uses ultrasonic oscillations and operates based on the propagation velocity of these oscillations in the air. Thus, we can easily determine the distance of two points if the time within which the wave travels this distance is....
Sometimes the precious metals are hidden too deep and are not detected except with complicated devices. In many cases, however, small pieces of precious metal buried near the surface can be detected by relatively simple means. Everyone is very attractive to....
Many times for various reasons we forget or can not water the plants that we have in our homes. And many humidity sensors units just notify us with a beeping sound or with a flashing light, that the pot needs watering. But what if we are away from home? This....
The principle behind a metal detector is really very simple. Proof of this is the circuit that follows, in which it proves that the construction of a metal detector can be done in the minute, with few components that we find very easily everywhere. With the....
The sub-harmonic bass generator is a sound producing unit for guitars. The sound it produces looks very much like that of the bass guitar. The octave generator is sometimes quite unusual since it does not produce any of the usual ways of coloring the sound,....
In the past the sounding (sea bottom measurement) was done with the "bullet", that is, with a heavy lead object that the seamen plucked into the sea hung from a calibrated rope. As soon as the "bullet" reached the bottom, the depth appeared directly from the....
This Amplifier was designed to have the following specifications: Distortion less than 0.1% at full power of 100W even at 20KHz. Power has to be attributed to an extended bandwidth. The output transistors must be protected against short circuits. The power....
A series of LEDs serve to alert the gardener when plants need water. Using two conventional digital integrated LEDs and a series of LEDs, we make a very useful device for gardening. The device detects the amount of water in the pot and alerts the grower.....
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