We present a simple and high-throughput microfluidic system for diffusion-based monolayer yeast cell culture monitoring. The technique takes advantage of gas solubility and elasticity of PDMS. Yeast cells are patterned into the micro-cavity array with a suitable height (4 m) that keeps the cells in monolayer during the cell division. As a demons
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tration, we apply this technology to investigate budding yeast and fission yeast cultures and show that it permits single-cell resolution over many cellular generations. Our results show that the technique provides an easy way to study the phenotype of single yeast cell cycle or cell-cell communication in high-throughput microfluidic system. Fig. 1 (a) Pattern for the2-layer microfluidic device fabrication. Layer 1: 100 square cavities with different side lengths (100, 125, 150, 175, 200 m); 100 square cavities with side lengths of 150 m. Layer 2: the mainchannel; (b) The top view and side view of the microfluidic device, Each cavity has a capillary of 40 m width ready to be connected to the mainchannel. (c) Procession of cells loaded into the cavity 2. Diffusion-based concentration control in microcavities during long time period by programmed syringe pumps, Guangwei si, Xuejun zhu, Yangsen Kang, Chunxiong Luo*, Qi Ouyang*, Yong Chen We propose a programmed syringe pumps system for diffusion-based concentration control in microcavities. Fluorescein is used to demonstrate the influence of different duration s of unvarying injecting speed and different sizes of microcavities on the concentration control and distribution in the microcavities. With longer duration of unvarying injecting speed and smaller size of microcavities, the concentration control of the microcavities will be more consistent with the input signal. Using...
This project fits more for custom made battery casing that can have more space to hide things inside. This switch with few alterations can be used for any other project as well but is designed based on e-bike needs! This project use a clever functionality of....
The circuit described here uses ultrasonic oscillations and operates based on the propagation velocity of these oscillations in the air. Thus, we can easily determine the distance of two points if the time within which the wave travels this distance is....
Sometimes the precious metals are hidden too deep and are not detected except with complicated devices. In many cases, however, small pieces of precious metal buried near the surface can be detected by relatively simple means. Everyone is very attractive to....
Many times for various reasons we forget or can not water the plants that we have in our homes. And many humidity sensors units just notify us with a beeping sound or with a flashing light, that the pot needs watering. But what if we are away from home? This....
The principle behind a metal detector is really very simple. Proof of this is the circuit that follows, in which it proves that the construction of a metal detector can be done in the minute, with few components that we find very easily everywhere. With the....
The sub-harmonic bass generator is a sound producing unit for guitars. The sound it produces looks very much like that of the bass guitar. The octave generator is sometimes quite unusual since it does not produce any of the usual ways of coloring the sound,....
In the past the sounding (sea bottom measurement) was done with the "bullet", that is, with a heavy lead object that the seamen plucked into the sea hung from a calibrated rope. As soon as the "bullet" reached the bottom, the depth appeared directly from the....
This Amplifier was designed to have the following specifications: Distortion less than 0.1% at full power of 100W even at 20KHz. Power has to be attributed to an extended bandwidth. The output transistors must be protected against short circuits. The power....
A series of LEDs serve to alert the gardener when plants need water. Using two conventional digital integrated LEDs and a series of LEDs, we make a very useful device for gardening. The device detects the amount of water in the pot and alerts the grower.....
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